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kdrfc chimeric protein  (R&D Systems)


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    R&D Systems kdrfc chimeric protein
    DARPin28z induces murine CAR-T cell cytokine production upon HER2 stimulation. a 10 6 scFv28z, DARPin28z, or CAR –’ve transduced murine T cells were stimulated with <t>HER2</t> <t>(HER2Fc</t> fusion protein) or an unrelated target <t>(KDRFc</t> fusion protein) for four hours at 37 °C in a 96-well plate. Production of IFN-γ and TNF-α was measured by intracellular cytokine staining (ICS) and subsequent flow cytometry. Data from CD8 + Thy1.1 + T cells is presented as mean of n = 3 experiments (CAR –’ve) and n = 5 experiments (scFv28z and DARPin28z) ± SD. Bracketed numbers are quantitative of the representative plots shown. b Visual comparison of cytokine production data from a. c Cytokine expression data expressed relative to CAR-positivity of transduced cells where CAR + transformant = % CAR + /% Thy1.1 + . Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001
    Kdrfc Chimeric Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kdrfc chimeric protein/product/R&D Systems
    Average 90 stars, based on 14 article reviews
    kdrfc chimeric protein - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors"

    Article Title: Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1186/s40425-015-0099-4

    DARPin28z induces murine CAR-T cell cytokine production upon HER2 stimulation. a 10 6 scFv28z, DARPin28z, or CAR –’ve transduced murine T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. Production of IFN-γ and TNF-α was measured by intracellular cytokine staining (ICS) and subsequent flow cytometry. Data from CD8 + Thy1.1 + T cells is presented as mean of n = 3 experiments (CAR –’ve) and n = 5 experiments (scFv28z and DARPin28z) ± SD. Bracketed numbers are quantitative of the representative plots shown. b Visual comparison of cytokine production data from a. c Cytokine expression data expressed relative to CAR-positivity of transduced cells where CAR + transformant = % CAR + /% Thy1.1 + . Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001
    Figure Legend Snippet: DARPin28z induces murine CAR-T cell cytokine production upon HER2 stimulation. a 10 6 scFv28z, DARPin28z, or CAR –’ve transduced murine T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. Production of IFN-γ and TNF-α was measured by intracellular cytokine staining (ICS) and subsequent flow cytometry. Data from CD8 + Thy1.1 + T cells is presented as mean of n = 3 experiments (CAR –’ve) and n = 5 experiments (scFv28z and DARPin28z) ± SD. Bracketed numbers are quantitative of the representative plots shown. b Visual comparison of cytokine production data from a. c Cytokine expression data expressed relative to CAR-positivity of transduced cells where CAR + transformant = % CAR + /% Thy1.1 + . Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001

    Techniques Used: Staining, Flow Cytometry, Comparison, Expressing

    DARPin28z induces human CAR-T cell cytokine production upon HER2 stimulation. 10 6 scFv28z, DARPin28z, or CAR –’ve transduced human T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. a Production of IFN-γ and TNF-α was measured by ICS and subsequent flow cytometry. Data from CD8 + NGFR + T cells is presented as mean ± SD. Bracketed numbers are quantitative of the representative plots shown. b Production of IL-2 by CD8 + NGFR + T cells as measured by ICS. c Production of CD107a by CD8 + NGFR + T cells as measured by ICS. d Pie graphs capturing the distribution of single and multi-functional CAR-T cells as produced with SPICE software. Pie arcs indicate functional populations represented by pie wedges. Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001. All data from n = 3 experiments repeated with T cells from two donors
    Figure Legend Snippet: DARPin28z induces human CAR-T cell cytokine production upon HER2 stimulation. 10 6 scFv28z, DARPin28z, or CAR –’ve transduced human T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. a Production of IFN-γ and TNF-α was measured by ICS and subsequent flow cytometry. Data from CD8 + NGFR + T cells is presented as mean ± SD. Bracketed numbers are quantitative of the representative plots shown. b Production of IL-2 by CD8 + NGFR + T cells as measured by ICS. c Production of CD107a by CD8 + NGFR + T cells as measured by ICS. d Pie graphs capturing the distribution of single and multi-functional CAR-T cells as produced with SPICE software. Pie arcs indicate functional populations represented by pie wedges. Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001. All data from n = 3 experiments repeated with T cells from two donors

    Techniques Used: Flow Cytometry, Functional Assay, Produced, Software



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    kdrfc chimeric protein  (R&D Systems)
    90
    R&D Systems kdrfc chimeric protein
    DARPin28z induces murine CAR-T cell cytokine production upon HER2 stimulation. a 10 6 scFv28z, DARPin28z, or CAR –’ve transduced murine T cells were stimulated with <t>HER2</t> <t>(HER2Fc</t> fusion protein) or an unrelated target <t>(KDRFc</t> fusion protein) for four hours at 37 °C in a 96-well plate. Production of IFN-γ and TNF-α was measured by intracellular cytokine staining (ICS) and subsequent flow cytometry. Data from CD8 + Thy1.1 + T cells is presented as mean of n = 3 experiments (CAR –’ve) and n = 5 experiments (scFv28z and DARPin28z) ± SD. Bracketed numbers are quantitative of the representative plots shown. b Visual comparison of cytokine production data from a. c Cytokine expression data expressed relative to CAR-positivity of transduced cells where CAR + transformant = % CAR + /% Thy1.1 + . Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001
    Kdrfc Chimeric Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kdrfc chimeric protein/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    kdrfc chimeric protein - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    DARPin28z induces murine CAR-T cell cytokine production upon HER2 stimulation. a 10 6 scFv28z, DARPin28z, or CAR –’ve transduced murine T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. Production of IFN-γ and TNF-α was measured by intracellular cytokine staining (ICS) and subsequent flow cytometry. Data from CD8 + Thy1.1 + T cells is presented as mean of n = 3 experiments (CAR –’ve) and n = 5 experiments (scFv28z and DARPin28z) ± SD. Bracketed numbers are quantitative of the representative plots shown. b Visual comparison of cytokine production data from a. c Cytokine expression data expressed relative to CAR-positivity of transduced cells where CAR + transformant = % CAR + /% Thy1.1 + . Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors

    doi: 10.1186/s40425-015-0099-4

    Figure Lengend Snippet: DARPin28z induces murine CAR-T cell cytokine production upon HER2 stimulation. a 10 6 scFv28z, DARPin28z, or CAR –’ve transduced murine T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. Production of IFN-γ and TNF-α was measured by intracellular cytokine staining (ICS) and subsequent flow cytometry. Data from CD8 + Thy1.1 + T cells is presented as mean of n = 3 experiments (CAR –’ve) and n = 5 experiments (scFv28z and DARPin28z) ± SD. Bracketed numbers are quantitative of the representative plots shown. b Visual comparison of cytokine production data from a. c Cytokine expression data expressed relative to CAR-positivity of transduced cells where CAR + transformant = % CAR + /% Thy1.1 + . Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001

    Article Snippet: 10 6 murine or human CAR-T cells were stimulated in round bottom tissue culture treated 96-well plates coated with 200 ng HER2Fc chimeric protein (Cat No. 1129-ER-050, R&D Systems) or 200 ng KDRFc chimeric protein (Cat No. 443-KD, R&D Systems) for 4 h at 37 °C.

    Techniques: Staining, Flow Cytometry, Comparison, Expressing

    DARPin28z induces human CAR-T cell cytokine production upon HER2 stimulation. 10 6 scFv28z, DARPin28z, or CAR –’ve transduced human T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. a Production of IFN-γ and TNF-α was measured by ICS and subsequent flow cytometry. Data from CD8 + NGFR + T cells is presented as mean ± SD. Bracketed numbers are quantitative of the representative plots shown. b Production of IL-2 by CD8 + NGFR + T cells as measured by ICS. c Production of CD107a by CD8 + NGFR + T cells as measured by ICS. d Pie graphs capturing the distribution of single and multi-functional CAR-T cells as produced with SPICE software. Pie arcs indicate functional populations represented by pie wedges. Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001. All data from n = 3 experiments repeated with T cells from two donors

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors

    doi: 10.1186/s40425-015-0099-4

    Figure Lengend Snippet: DARPin28z induces human CAR-T cell cytokine production upon HER2 stimulation. 10 6 scFv28z, DARPin28z, or CAR –’ve transduced human T cells were stimulated with HER2 (HER2Fc fusion protein) or an unrelated target (KDRFc fusion protein) for four hours at 37 °C in a 96-well plate. a Production of IFN-γ and TNF-α was measured by ICS and subsequent flow cytometry. Data from CD8 + NGFR + T cells is presented as mean ± SD. Bracketed numbers are quantitative of the representative plots shown. b Production of IL-2 by CD8 + NGFR + T cells as measured by ICS. c Production of CD107a by CD8 + NGFR + T cells as measured by ICS. d Pie graphs capturing the distribution of single and multi-functional CAR-T cells as produced with SPICE software. Pie arcs indicate functional populations represented by pie wedges. Error bars = SD. * = p < 0.05 ** = p < 0.005 *** = p < 0.001. All data from n = 3 experiments repeated with T cells from two donors

    Article Snippet: 10 6 murine or human CAR-T cells were stimulated in round bottom tissue culture treated 96-well plates coated with 200 ng HER2Fc chimeric protein (Cat No. 1129-ER-050, R&D Systems) or 200 ng KDRFc chimeric protein (Cat No. 443-KD, R&D Systems) for 4 h at 37 °C.

    Techniques: Flow Cytometry, Functional Assay, Produced, Software